Phase II Research – Advanced Absorption Of Crucial Nutrients
Single Dose Pharmacokinetic Study of D-Glucosamine HCl In Sprague Dawley Rats by Oral (Gavage) Administration
Study summary
Green Gruff Veterinary increased the absorption of glucosamine into the blood plasma and synovial fluid more effectively than glucosamine alone.
We conducted a clinical trial to understand how Green Gruff Veterinary increases the absorption of glucosamine into the plasma and synovial fluid. An experimental and control group of rats were administered Green Gruff Veterinary or glucosamine alone, respectively. This study had 2 main findings:- After 8 hours, the concentration of glucosamine in the blood plasma was 207% greater in the experimental group than the control group.
- There was a higher concentration of glucosamine in the synovial fluid of the experimental group than the control group.
Study outline
Groups:
| Groups | Treatment | № of animals |
|---|---|---|
| G1 | Green Gruff Veterinary | 7 M |
| G2 | Glucosamine (Gln) | 7 M |
Gln dose: 500 mg/Kg
Total administration volume: 10 mL
Blood testing for Gln concentration: Blood was collected at 1, 2, 4 and 8 hours post dosing in tubes containing K2/K3-EDTA.
Synovial fluid collection: 8 hours post dosing.
Plasma and synovial fluid samples were processed using acetonitrile precipitation and analysed by HPLC-MS/MS.
Gln plasma concentration (ng/mL), 1, 2, 4 and 8 hours post administration
A significantly higher Gln-plasma concentration was observed in animals administered Green Gruff Veterinary compared to those administered Gln alone.
| Increase by % | |
|---|---|
| 1 hour | 25.6% |
| 2 hours | 36.2% |
| 4 hours | 14.4% |
| 8 hours | 207.1% |
Gln concentration in the synovial fluid (ng/mL) 8 hours post administration
A higher Gln concentration in the synovial fluid was observed in animals administered Green Gruff Veterinary compared to those administered Gln alone despite high variance among the animals.
